Atypical sympathetic arousal in children with autism spectrum disorder and its association with anxiety symptomatology
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Aliquots of the cDNA were used to measure the expression levels of the genes with the primers, and Power SYBR Green master mix (Applied Biosystems, ThermoFisher Scientific) on a 7900 HT sequence detection system (Applied Biosystems, ThermoFisher Scientific)
In brief, 5-m paraffin-embedded tissue sections were deparaffinized, fixed with 4% PFA for 5 min, and permeabilized with 0.5% Triton X-100