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doi: 10.7759/cureus.40357 149

Molecular pathways: tumor cells co-opt the brain-specific metabolism gene CPT1C to promote survival

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Common Enzymes: Trypsin (most widely used for predictable cleavage) Chymotrypsin (targets aromatic residues) Glu-C (cleaves at acidic residues) Challenges: Incomplete digestion leading to reduced sequence coverage Overdigestion causing excessively small fragments Missed cleavage sites affecting data interpretation 3