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The next day, media was exchanged with 200 L of DMEM +dFBS supplemented with 300 ng/mL of Dox and other components as indicated (for hypoxia experiments, microplates were transferred to an incubator set to 0.5% O 2 or 3.5% O 2 )

In endothelium-denuded study, a cotton stick was used to gently remove the endothelium by rubbing the intimal surface of vessel

Proper storage and handling help reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation, which can affect experimental reliability

Lipohypertrophy: prevalence, clinical consequence, and pathogenesis

Jafar AB, Jacob J, Kao WK, Ho T

However, you should avoid using heavily scented antibacterial soaps directly on the red area, as the fragrances and harsh chemicals can worsen the irritation